报告题目：Imaging molecules and cells at high spatiotemporal resolution throughout whole living organisms

报 告 人：Kai Wang,Janelia Research Campus, Howard Hughes Medical Institute

报告时间：2015年5月25日10：30

报告地点：理科楼三楼报告厅（C302）

报告摘要：Much of our knowledge of the structural/functional relationships within cells comes from images of immortalized cells cultured on glass substrates. This obviously artificial setting is chosen because it is tractable for standard research-grade microscopes. Imaging at diffraction-limited resolution or even superresolution in the more relevant context of whole living organisms, where cell-cell interactions play crucial roles, is far more difficult, due to the heterogeneity of cell morphologies within tissues and the additional heterogeneity of sub-cellular domains within each cell. These cause the light rays that normally converge to a sharp focus to instead warp in unpredictable ways, producing an extended, aberrated excitation region that yields images of low resolution, reduced signal, and high background. To address this issue, I have developed an adaptive optical microscope to correct sample induced aberrations dynamically, providing high resolution images with large field of views at depth. After compensating the aberration, we demonstrated in vivo high resolution imaging over large volumes in the brains of zebrafish, fruit fly, and mouse. This technique can be further combined with our recently developed lattice light sheet microscope to enable high resolution, fast and minimal invasive imaging of molecules and cells in whole living organisms.