报告题目：Single-molecule Biophysical View of Gene Expression and Regulation

报 告 人：Jingyi Fei，Center for the Physics of Living Cells / Taekjip Ha Group, Department of Physics, University of Illinois Urbana-Champaign

报告时间：12月17日（周一） 10:30

报告地点：理科楼物理系三楼报告厅

报告摘要：Gene expression is one of the most fundamental and essential biological processes in living cells. Nowadays single-molecule and single-cell biophysical approaches have provided powerful means in studying gene expression and regulation. In the first part of my talk, I will illustrate the application of single-molecule fluorescent resonance transfer in revealing critical conformational dynamics of translating ribosome, one of the largest cellular machines responsible for protein synthesis. In the second part, I will demonstrate a potential new application of the single-molecule fluorescence-based super resolution imaging technique in the quantitative analysis of small RNA-mediated gene regulation at the cellular level.

Ref:

1. Fei, J., Kosuri, P., MacDougall, D. and Gonzalez, R.L., Jr. (2008). Coupling of ribosomal L1 stalk and tRNA dynamics during translation elongation. Mol Cell, 30: 348-359.

2. Fei, J., Bronson, J.E., Hofman, J.M., Srinivas, R.L., Wiggins, C.H. and Gonzalez, R.L., Jr. (2009). Allosteric collaboration between elongation factor G and the ribosomal L1 stalk directs tRNA movements during translation. Proc Natl Acad Sci, USA, 106:15702-15707.

3. Fei, J., Richard, A.C., Bronson, J.E. and Gonzalez, R.L., Jr. (2011). Transfer RNA-mediated control of ribosome dynamics during translation. Nat Struct Mol Biol 18:1043-51.